MAVS is Important for Antiviral Defense Against InfluenzaA Virus in a Human Respiratory Epithelium Model
Hemberg, M.; Hansen, A. L.; Storgaard, J.; Blay-Cadanet, J.; Pedersen, A.; Thielke, A. L.; Holm, C. K.
Show abstract
The respiratory epithelium is an important immunological barrier and the first line of defense against influenza A virus. In mice and in various cellular systems, induction of type I interferons (IFN/{beta}) during IAV infections is known to depend on cytosolic RNA sensors RIG-I and MDA5 and on the adaptor molecule MAVS. Until now, it has not been possible to directly test the importance of MAVS for induction of IFNs and for resistance to IAV infection in primary human respiratory epithelium. Here, we used CRISPR-Cas9 to establish MAVS-deficient cultures of primary human respiratory epithelium using the air-liquid interphase culture system. Using this setup, we show that MAVS is indeed required for the induction of type I and type III IFNs and subsequently for the induction of IFN-stimulated genes in response to IAV infection in human primary respiratory epithelium. Finally, we demonstrate that MAVS is important for restricting viral replication in this model. In conclusion, we demonstrate that MAVS plays a non-redundant protective role during IAV infection in primary human respiratory epithelium.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- The SARS-CoV-2 multibasic cleavage site facilitates early serine protease-mediated entry into organoid-derived human airway cells 96%
- Murine Alveolar Macrophages Rapidly Accumulate Intranasally Administered SARS-CoV-2 Spike Protein leading to Neutrophil Recruitment and Damage 94%
- Identification of scavenger receptor B1 as the airway microfold cell receptor for Mycobacterium tuberculosis 93%
Similar papers in this journal
- Stealth replication of SARS-CoV-2 Omicron in the nasal epithelium at physiological temperature 96%
- SARS-CoV-2 replication triggers an MDA-5-dependent interferon production which is unable to efficiently control replication 96%
- Human nasal and lung tissues infected ex vivo with SARS-CoV-2 provide insights into differential tissue-specific and virus-specific innate immune responses in the upper and lower respiratory tract 95%
Similar papers in this journal
- Glycosylated extracellular mucin domains protect against SARS CoV-2 infection at the respiratory surface 96%
- Rhinovirus C replication is associated with the endoplasmic reticulum and triggers cytopathic effects in an in vitro model of human airway epithelium 95%
- Viral interference between severe acute respiratory syndrome coronavirus 2 and influenza A viruses 95%
Similar papers in this journal
- The purinergic receptor P2X7 and the NLRP3 inflammasome are druggable host factors required for SARS-CoV-2 infection 94%
- Differential Immunoregulation by Human Surfactant Protein A Variants Determines Severity of SARS-CoV-2-induced Lung Disease 94%
- A Murine Model of Post-acute Neurological Sequelae Following SARS-CoV-2 Variant Infection 93%
Similar papers in this journal
- Long Period Modeling SARS-CoV-2 Infection of in Vitro Cultured Polarized Human Airway Epithelium 97%
- The human nose organoid respiratory virus model: an ex-vivo human challenge model to study RSV and SARS-CoV-2 pathogenesis and evaluate therapeutics 96%
- Comparison of SARS-CoV-2 variants of concern in primary human nasal cultures demonstrates Delta as most cytopathic and Omicron as fastest replicating 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.