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Anatomy and mechanics of tsetse fly blood feeding

Stephan, L.; Hauf, L.; Meyer-Natus, E.; Katiti, D.; Petersen, D.; Kovalev, A.; Büsse, S.; Steyer, A.; Matsumura, Y.; Böhme, W.; Masiga, D.; Gorb, S.; Engstler, M.

2025-12-11 biophysics
10.64898/2025.12.08.692967 bioRxiv
Show abstract

African trypanosomiasis is a neglected tropical disease transmitted by tsetse flies (Glossina spp.) in sub-Saharan Africa. The flys saliva carries parasitic unicellular trypanosomes, such as Trypanosoma brucei, leading to infections in humans, domestic animals, and wildlife. Tsetse flies feed on a broad spectrum of hosts, including humans, elephants, buffaloes, rhinos, hippos, turtles and monitor lizards. To understand how the insects can penetrate such diverse skin types, we detailed the anatomical structures involved in tsetse blood feeding, their mechanical properties and the forces exerted by the fly during probing. We found that the tsetse flys feeding apparatus does not rely on exceptionally unique structures or extraordinary forces. Instead, the tsetse fly has evolved subtle yet highly efficient adaptations, which include a labellum equipped with arrays of small teeth. When combined with a robust retraction movement of the proboscis, these structures create lesions on various types of skin, thus facilitating blood pool feeding on a broad host spectrum.

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