Plasmid copy number affects the DNA methylation-driven expression dynamics of the CfrBI restriction-modification system and impacts phage restriction
Gibhardt, J.; Hoang, T. D.; Severinov, K.; Khodorkovskii, M. A.; Morozova, N. E.
Show abstract
Restriction-modification (R-M) systems are one of the most widespread and, due to their often plasmid-based nature, transmittable anti-phage systems bacteria have. The CfrBI R-M system studied here consists of a methyltransferase (MT) and a restriction endonuclease (RE) that are divergently expressed and share a promoter region that harbors a single CfrBI recognition site. Previously, the methylation of this site has been shown to regulate the expression of the R-M system. Here, we show that the expression dynamics of the CfrBI R-M system and its protective properties depend on the copy number of the plasmid harboring it. A higher copy number results in higher expression, but the expression on a medium-copy number plasmid interestingly conferred the highest phage resistance. After transformation of naive cells however, expression of the RE was fastest in the high-copy plasmid background. In vivo we show that the expression strength of the MT inhibits its own expression, while enhancing RE expression. To conclude, the results indicate that for phage resistance the overall expression strength might not be the predominant factor in case of the CfrBI R-M system, while for the establishment of the system the initial expression rate of the MT seems to be the determining factor.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Cells with Stochastically Increased Methyltransferase to Restriction Endonuclease Ratio Provide an Entry for Bacteriophage into Protected Cell Population 97%
- Correction of non-random mutational biases along a linear bacterial chromosome by the mismatch repair endonuclease NucS 96%
- DNA modifications impact natural transformation of Acinetobacter baumannii 95%
Similar papers in this journal
- Single molecule dynamics of DNA receptor ComEA, membrane permease ComEC and taken up DNA in competent Bacillus subtilis cells 96%
- High abundance of transcription regulators compacts the nucleoid in Escherichia coli 96%
- Staphylococcus aureus does not synthesize arginine from proline under physiological conditions 95%
Similar papers in this journal
- Lysis cassette-mediated exoprotein release in Yersinia entomophaga is controlled by a PhoB-like regulator 96%
- Conjugative IncC plasmid entry triggers the SOS response and promotes effective transfer of the integrative antibiotic resistance element SGI1 96%
- Expression of the Bacillus thuringiensis vip3A insecticidal toxin gene is activated at the onset of stationary phase by VipR, an autoregulated transcription factor 96%
Similar papers in this journal
- Comparative analysis of MOBQ4 plasmids demonstrates that MOBQ is a cis-acting-enriched relaxase protein family 96%
- The LAMP-CRISPR-Cas13a technique for detecting CBASS-mechanism of phage resistance in bacteria 94%
- Soft rot pathogen Dickeya dadantii 3937 produces tailocins resembling the tails of Enterobacteria bacteriophage P2 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.