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Dynamic alterations of DNA methylation and transcriptome in adaptation to and recovery from the space environment

Lu, L.; Hao, Y.; Lin, X.; Li, K.; Zhai, T.; Liang, F.; Chen, L.; Wang, L.; Mei, X.; Feng, S.; Lv, K.; Yuan, Y.; Dai, Z.; Liu, D.; Zhang, H.; Yang, C.; Liu, A.; Wang, L.; Li, Z.; Liu, S.; Dai, X.; Yang, C.; Wang, C.; Sun, P.; Shi, L.; Zhang, C.; Xiong, J.; Wei, M.; Xu, C.; Liu, Z.; Qu, L.; Li, J.; Li, Y.

2025-12-05 genomics
10.64898/2025.12.03.691098 bioRxiv
Show abstract

Space exploration presents tremendous health challenges. Here, we report time series of multi-omic and phenotypic profiles of seventeen astronauts from six China Manned Space missions with continuous spaceflight durations ranging from 13 to 180 days. We revealed a key role of DNA methylation regulation in reshaping gene expression patterns to adapt to the space environment. Long-duration spaceflight showed more alterations in epigenetic modifications correlated with alternative splicing and protein acetylation. During recovery, an "overrange rebound" phenomenon was observed, furthermore, a mathematical model was established to describe this implying important phenomenon. Moreover, we revealed the correlations between molecular alterations and phenotypic changes such as coagulation activation and bone intensity loss. Additionally, we performed ground-based simulation experiments to estimate the impacts of individual stressors in the space environment on DNA methylation. In summary, our study highlights the importance and complexity of epigenetic regulation in adaptation to and recovery from the space environment.

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