A developmentally regulated long-range enhancer-promoter contact mediates human neural development
Bready, D.; Wang, S.; Ravn-Boess, N.; Frenster, J.; Sabio, J.; Kushmakov, R.; Clark, F.; Guerrero, A.; Lapierre, C.; Galbraith, K.; Do, C.; Lhoumaud, P.; Prado, J.; Jiang, A.; Haddock, S.; Kim, C. D.; Snuderl, M.; Lionnet, T.; Tsirigos, A.; Skok, J.; Placantonakis, D. G.
Show abstract
SOX2 is a core pluripotency factor in human embryonic stem cells (hESCs), but upon differentiation to the three germ layers, its expression is preserved selectively in neuroectoderm. The mechanisms regulating SOX2 transcription in distinct developmental stages remain incompletely understood. Here, we demonstrate that a distant enhancer 550 kb from the human SOX2 locus is selectively activated in neural stem cells (NSCs) and establishes long-range contact with the SOX2 gene. CRISPR-Cas9 excision of the enhancer has no effect in hESCs but reduces SOX2 transcription in NSCs and impairs neuroectodermal differentiation and forebrain specification in teratomas and cerebral organoids. CRISPR excision of a CTCF recognition motif adjacent to the enhancer does not affect enhancer activation in neuroectoderm but reduces chromatin looping and SOX2 transcription to partially reproduce phenotypes seen with enhancer deletion. Our findings indicate that the development of the human nervous system depends on a developmentally regulated long-range contact between a distant enhancer and the SOX2 locus.
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