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Need-selective gating of dopamine neuron cue responses by real and virtual hunger

Mamaligas, A. A.; Berke, J.; Kreitzer, A. C.; Knight, Z. A.

2025-12-02 neuroscience
10.64898/2025.11.29.691326 bioRxiv
Show abstract

The midbrain dopamine system is important for linking reward-predictive cues to learning and 20 motivation. Here we investigated how dopamine neuron responses to food and water cues are modulated by changes in internal state. We developed a flexible cued-approach task that allowed us to examine behavioral and neural responses to both food- and water-predictive cues within the same recording session. We found that overlapping subsets of dopamine neurons respond to food and water cues, but that the magnitude of these responses is gated in a need-25 specific way. Stimulation of hunger-promoting AgRP neurons amplified dopamine neuronresponses to food cues, but not water cues, and the magnitude of these responses exceeded those observed in natural hunger. These findings indicate that changes in internal state modulate, in a need-appropriate way, the responses of a common set of dopamine neurons to environmental signals of food and water availability.

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