Towards a scaled-up T cell-mediated cytotoxicity assay in 3D cell culture using microscopy
Gottschalk, E.; Czech, E.; Aksoy, B. A.; Aksoy, P.; Hammerbacher, J.
Show abstract
Three-dimensional (3D) cell culture systems with tumor spheroids are being adopted for research on the antitumor activity of drug treatments and cytotoxic T cells. Analysis of the cytotoxic effect on 3D tumor cultures within a 3D scaffold, such as collagen, is challenging. Image-based approaches often use confocal microscopy, which greatly limits the sample size of tumor spheroids that can be assayed. We explored a system where tumor spheroids growing in a collagen gel within a microfluidics chip can be treated with drugs or co-cultured with T cells. We attempted to adapt the system to measure the death of cells in the tumor spheroids directly in the microfluidics chip via automated widefield fluorescence microscopy. We were able to successfully measure drug-induced cytotoxicity in tumor spheroids, but had difficulties extending the system to measure T cell-mediated tumor killing. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=193 SRC="FIGDIR/small/842039v1_ufig1.gif" ALT="Figure 1"> View larger version (44K): org.highwire.dtl.DTLVardef@c95725org.highwire.dtl.DTLVardef@785728org.highwire.dtl.DTLVardef@a23dceorg.highwire.dtl.DTLVardef@187bd74_HPS_FORMAT_FIGEXP M_FIG C_FIG
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Real-time integration of cell death mechanisms and proliferation kinetics at the single-cell and population-level using high-throughput live-cell imaging. 90%
- An extensively optimized chromatin immunoprecipitation protocol for quantitatively comparable and robust results 89%
- Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation 88%
Similar papers in this journal
- Automated workflow for the cell cycle analysis of non-adherent and adherent cells using a machine learning approach 92%
- Extracellular ATP drives pancreatic cancer cell invasion via purinergic receptor-integrin interactions 92%
- Complementary CRISPR screen highlights the contrasting role of membrane-bound and soluble ICAM-1 in regulating antigen specific tumor cell killing by cytotoxic T cells 91%
Similar papers in this journal
- A Simplified Function-First Method for the Discovery and Optimization of Bispecific Immune Engaging Antibodies 92%
- A novel Fiji/ImageJ plugin for the rapid analysis of blebbing cells 92%
- High-volume, label-free imaging for quantifying single-cell dynamics in induced pluripotent stem cell colonies 91%
Similar papers in this journal
- Towards a More Objective and High-throughput Spheroid Invasion Assay Quantification Method 92%
- HiIDDD: A high-throughput imaging pipeline for the quantitative detection of DNA damage in primary human immune cells 92%
- Drug resistant pancreatic cancer cells exhibit altered biophysical interactions with stromal fibroblasts in imaging studies of 3D co-culture models 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.