Expansion Microscopy of Lipid Membranes
Karagiannis, E. D.; Kang, J. S.; Shin, T. W.; Emenari, A.; Asano, S.; Lin, L.; Costa, E. K.; CRUK IMAXT Grand Challenge Consortium, ; Marblestone, A. H.; Kasthuri, N.; Boyden, E. S.
Show abstract
Lipids are fundamental building blocks of cells and their organelles, yet nanoscale resolution imaging of lipids has been largely limited to electron microscopy techniques. We introduce and validate a chemical tag that enables lipid membranes to be imaged optically at nanoscale resolution via a lipid-optimized form of expansion microscopy, which we call membrane expansion microscopy (mExM). mExM, via a novel post-expansion antibody labeling protocol, enables protein-lipid relationships to be imaged in organelles such as mitochondria, the endoplasmic reticulum, the nuclear membrane, and the Golgi apparatus. mExM may be of use in a variety of biological contexts, including the study of cell-cell interactions, intracellular transport, and neural connectomics.
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