Translationally relevant transcriptomic alterations in mouse ischemic cerebral microvessels
Callegari, K.; Dash, S.; Uchida, H.; Lee, Y.; Ito, A.; Zhang, T.; Xiang, J.; Sanchez, T.
Show abstract
Increasing evidence implicates cerebral microvascular dysfunction in the pathophysiology of numerous central nervous system pathologies, including stroke. Understanding the molecular alterations in cerebral microvessels in these conditions will provide original opportunities for scientific investigation at the pre-clinical and clinical levels. In this study, we conducted a novel genome-wide transcriptomic analysis of microvessels in a mouse model of transient focal cerebral ischemia. Using a publicly available human ischemic stroke dataset, we identified shared alterations in our microvessel dataset with implications for human pathophysiology. From this unbiased analysis, we report predicted alterations in inter- and intra-cellular signaling, emphasizing perturbations in genes involved in blood brain barrier function, endothelial cell activation and metabolism. Furthermore, our study unveiled previously unreported gene expression changes associated with altered sphingolipid metabolism. Altogether, our results have identified microvessel-specific transcriptomic changes in a number of translationally relevant pathways that support the targeting of these pathways in preclinical studies. The data shared here provide a resource for future investigation of translationally relevant pathways in ischemic stroke.
Matching journals
The top 17 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Chronic Changes In Oligodendrocyte Sub-Populations After Middle Cerebral Artery Occlusion in Neonatal Mice. 95%
- Spatial Transcriptomic Analysis Reveals HDAC Inhibition Modulates Microglial Dynamics to Protect Against Ischemic Stroke in Mice 95%
- Specific deletion of interleukin-1 beta in microglia improves acute outcome and modulates neurogenesis after ischemic stroke 92%
Similar papers in this journal
- Astrocyte-secreted chordin-like 1 regulates spine density after ischemic stroke 95%
- Locational memory of macrovessel vascular cells is transcriptionally imprinted 93%
- Optimized Enrichment of Murine Blood-Brain Barrier Vessels with a Critical Focus on Network Hierarchy in Post-Collection Analysis 93%
Similar papers in this journal
- A Role For Astrocytic Insulin-Like Growth Factor I Receptors In The Response To Ischemic Insult 95%
- RNF213 loss of function reshapes vascular transcriptome and spliceosome leading to disrupted angiogenesis and aggravated vascular inflammatory responses. 94%
- Increased capillary stalling is associated with endothelial glycocalyx loss in subcortical vascular dementia 93%
Similar papers in this journal
- Vasa vasorum lumen narrowing in brain vascular hyalinosis in systemic hypertension patients with ischemic stroke 96%
- Epidermal growth factor receptors in vascular endothelial cells contribute to functional hyperemia in the brain 95%
- The Rho/Rac guanine nucleotide exchange factor Vav1 regulates HIF-1α; and GLUT-1 expression and glucose uptake in the brain 93%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.