Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. coli
Rajagopalan, K.; Dworkin, J.
Show abstract
In bacteria, signaling phosphorylation is thought to occur primarily on His and Asp residues. However, phosphoproteomic surveys in phylogenetically diverse bacteria over the past decade have identified numerous proteins that are phosphorylated on Ser and/or Thr residues. Consistently, genes encoding Ser/Thr kinases are present in many bacterial genomes such as E. coli, which encodes at least three Ser/Thr kinases. Here we identify a previously uncharacterized ORF, yegI, and demonstrate that it encodes a novel Ser/Thr kinase. YegI lacks several conserved residues including those important for Mg2+ binding seen in other bacterial Ser/Thr kinases, suggesting that the consensus may be too stringent. We further find that YegI is a two-pass membrane protein with both N- and C-termini located intracellularly.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Function and structure of FlaK, a master regulator of the polar flagellar genes in marine Vibrio 95%
- CwlQ is required for swarming motility but not flagellar assembly in Bacillus subtilis 94%
- Phosphorylation on PstP regulates cell wall metabolism and antibiotic tolerance in Mycobacterium smegmatis 94%
Similar papers in this journal
- A Legionella effector kinase is activated by host inositol hexakisphosphate 95%
- YhcB (DUF1043), a novel cell division protein conserved across gamma-proteobacteria 94%
- Cyclic AMP competitively inhibits periplasmic phosphatases to coordinate nutritional growth with competence development of Haemophilus influenzae 94%
Similar papers in this journal
- A TPR scaffold couples signal detection to OdhI phosphorylation in metabolic control by the protein kinase PknG 95%
- Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion system 94%
- An essential membrane protein modulates the proteolysis of LpxC to control lipopolysaccharide synthesis in Escherichia coli 94%
Similar papers in this journal
Similar papers in this journal
- Cytosolic factors controlling PASTA kinase-dependent ReoM phosphorylation 96%
- Autophosphorylation of the KaiC-like protein ArlH inhibits oligomerisation and interaction with ArlI, the motor ATPase of the archaellum 94%
- Phosphorylation chemistry of the Bordetella PlrSR TCS and its contribution to bacterial persistence in the lower respiratory tract 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.