kSHREC ‘Delta’ reflects the shape of kinetochore rather than intrakinetochore tension
Renda, F.; Magidson, V.; Tikhonenko, I.; Miles, C. E.; Mogilner, A.; Khodjakov, A.
Show abstract
Distance between fluorescent spots formed by various kinetochore proteins ( Delta) is proposed to reflect the level of intrakinetochore tension (IKT). However, larger-scale changes in the kinetochore architecture may also affect Delta. To test this possibility, we measure Delta in long kinetochores of Indian muntjac (IM) whose shape, size, and orientation are discernable in conventional light microscopy. We find that architecture of IM kinetochores and the value of Delta change minimally when microtubule-mediated forces are suppressed by Taxol. In contrast, large decreases of Delta observed in Taxol-treated human cells coincide with prominent changes in length and shape of the kinetochore. We also find that inner and outer kinetochore proteins intermix within a common spatial compartment instead of forming separate thin layers. These observations, supported by computational modelling, suggest that changes in Delta reflect changes in the kinetochore shape rather than the level of IKT.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Atypical and distinct microtubule radial symmetries in the centriole and the axoneme of Lecudina tuzetae. 94%
- FAZ assembly in bloodstream form Trypanosoma brucei requires kinesin KIN-E 93%
- An unbiased, quantitative and versatile method for determining misaligned and lagging chromosome during mitosis 93%
Similar papers in this journal
Similar papers in this journal
- Optogenetic control of PRC1 reveals that bridging fibers promote chromosome alignment by overlap length-dependent forces 94%
- Augmin prevents merotelic attachments by promoting proper arrangement of bridging and kinetochore fibers 94%
- A delta-tubulin/epsilon-tubulin/Ted protein complex is required for centriole architecture 94%
Similar papers in this journal
- CSAG1 Maintains the Integrity of the Mitotic Centrosome in Cells with Defective p53 93%
- Identification of locally activated spindle-associated proteins in oocytes uncovers a phosphatase-driven mechanism 93%
- AURKA destruction is decoupled from its activity at mitotic exit but suppresses interphase activity 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.