Significant improvement of reprogramming efficiency by transient overexpression of ZGA inducer Dux but not Dppa2/4
Yang, L.; Liu, X.; Song, L.; Di, A.; Su, G.; Bai, C.; Wei, Z.; Li, G.
Show abstract
Cloned animal have been reported by somatic cell nuclear transfer (SCNT) for many years. However, the SCNT is extremely inefficient, and zygotic genome activation (ZGA) is required for SCNT and chemical mediated reprogramming. To identify candidate factors that facilitate ZGA in reprogramming, we performed siRNA-repressor and mRNA-inducer screening, which revealed Dux, Dppa2, and Dppa4 as key factors enhancing the ZGA in SCNT. Direct injection of ZGA-inducers had no significant effect on the SCNT blastocyst formation, and even destroyed the ZGA. Through a inducible Dux transgenic mouse model, we demonstrate that transient overexpression of Dux not only improved SCNT efficiency, but also increased the efficiency of chemical reprogramming. Transcriptome profiling revealed that Dux treated SCNT embryos were similar to fertilized embryos. Furthermore, transient overexpression of Dux combined with inactivation of DNA methyltransferases (Dnmts) further promote the overall development of SCNT-derived animals. These findings enhance our understanding of ZGA-regulators in somatic reprogramming.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Endosomal-lysosomal organellar assembly (ELYSA) structures coordinate lysosomal degradation systems through mammalian oocyte-to-embryo transition 94%
- Directed Differentiation of Human iPSCs to Functional Ovarian Granulosa-Like Cells via Transcription Factor Overexpression 93%
- Study on gene knockout mice and human mutant individual reveals absence of CEP78 causes photoreceptor and sperm flagella impairments 93%
Similar papers in this journal
- Catalytic inhibition of H3K9me2 writers disturbs epigenetic marks during bovine nuclear reprogramming. 94%
- The polymicrogyria-associated GPR56 promoter preferentially drives gene expression in developing GABAergic neurons in common marmosets 93%
- Characterization of mitochondrial health from human peripheral blood mononuclear cells to cerebral organoids derived from induced pluripotent stem cells 93%
Similar papers in this journal
- Temporal regulation of prenatal embryonic development by paternal imprinted loci 94%
- Surrogate production of genome edited sperm from a different subfamily by spermatogonial stem cell transplantation 92%
- Plat safeguards maternally aged oocytes against programmed cell death through activating the Erk1/2 pathway 92%
Similar papers in this journal
- Immunity-and-Matrix-Regulatory Cells Derived from Human Embryonic Stem Cells Safely and Effectively Treat Mouse Lung Injury and Fibrosis 93%
- Modeling Post-Gastrula Development via Bidirectional Pluripotent Stem Cells 92%
- Dissecting endothelial to haematopoietic stem cell transition by single-cell transcriptomic and functional analyses 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.