L1 and B1 repeats blueprint the spatial organization of chromatin
Lu, J. Y.; Chang, L.; Li, T.; Sun, Y.; Shen, X.
Show abstract
Despite extensive mapping of three-dimensional (3D) chromatin structures, the basic principles underlying genome folding remain unknown. Here, we report a fundamental role for L1 and B1 retrotransposons in shaping the macroscopic 3D genome structure. Homotypic clustering of B1 and L1 repeats in the nuclear interior or at the nuclear and nucleolar peripheries, respectively, segregates the genome into mutually exclusive nuclear compartments. This spatial segregation of L1 and B1 is conserved in mouse and human cells, and occurs dynamically during establishment of the 3D chromatin structure in early embryogenesis and the cell cycle. Depletion of L1 transcripts drastically disrupts the spatial distributions of L1- and B1-rich compartments. L1 transcripts are strongly associated with L1 DNA sequences and induce phase separation of the heterochromatin protein HP1. Our results suggest that genomic repeats act as the blueprint of chromatin macrostructure, thus explaining the conserved higher-order structure of chromatin across mammalian cells.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Trading Genome Vulnerability for Stable Genetic Inheritance: Active Retrotransposons Help Maintain Pericentromeric Heterochromatin Required for Faithful Cell Division 97%
- Bivalent chromatin protects reversibly repressed genes from irreversible silencing 96%
- Histone marks retained during epigenetic reprogramming and their roles essential for fish early development 96%
Similar papers in this journal
- Chromosome Compartments on the Inactive X Guide TAD Formation Independently of Transcription during X-Reactivation 96%
- RTEL1 Influences the Abundance and Localization of TERRA RNA 96%
- Binding of guide piRNA triggers methylation of the unstructured N-terminal region of Aub leading to assembly of the piRNA amplification complex 95%
Similar papers in this journal
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.