Differential Effects of SUMO1/2 on Circadian Protein PER2 Stability and Function
Chen, L.-C.; Hsieh, Y.-L.; Kuo, T.-Y.; Chou, Y.-C.; Hsu, P.-H.; Hwang-Verslues, W. W.
Show abstract
Posttranslational modification (PTM) of core circadian clock proteins, including Period2 (PER2), is required for proper circadian regulation. PER2 function is regulated by casein kinase 1 (CK1)-mediated phosphorylation and ubiquitination but little is known about other PER2 PTMs or their interaction with PER2 phosphorylation. We found that PER2 can be SUMOylated by both SUMO1 and SUMO2; however, SUMO1 versus SUMO2 conjugation had different effects on PER2 turnover and transcriptional suppressor function. SUMO2 conjugation facilitated PER2-{beta}-TrCP interaction leading to PER2 proteasomal degradation. In contrast, SUMO1 conjugation, mediated by E3 SUMO-protein ligase RanBP2, enhanced CK1-mediated PER2S662 phosphorylation and increased PER2 transcriptional suppressor function. PER2 K736 was critical for both SUMO1- and SUMO2-conjugation. A PER2K736R mutation was sufficient to alter circadian periodicity and reduce PER2-mediated transcriptional suppression. Together, our data revealed SUMO1 versus SUMO2 conjugation acts as an upstream determinant of PER2 phosphorylation and thereby affects the circadian regulatory system and circadian periodicity.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Gain-of-function genetic screen of the kinome reveals BRSK2 as an inhibitor of the NRF2 transcription factor 95%
- Intramembrane protease RHBDL4 cleaves oligosaccharyltransferase subunits to target them for ER-associated degradation 93%
- ACSL3 is a novel GABARAPL2 interactor that links ufmylation and lipid droplet biogenesis 93%
Similar papers in this journal
- Differential transcriptional activity of ΔNp63β is encoded by an isoform-specific C-terminus 93%
- Histone chaperone Nucleophosmin regulates transcription of key genes involved in oral tumorigenesis. 92%
- NRF1 and NRF3 complementarily maintain a basal proteasome activity in cancer cells through CPEB3-mediated translational repression 91%
Similar papers in this journal
- Aurora Kinase A proximity interactome reveals centriolar satellites as regulators of its function during primary cilium biogenesis 94%
- Circadian oscillation in primary cilium length by clock genes regulate fibroblast cell migration 94%
- Activation of FAM111A Protease Induces Defects in Nuclear Function that Likely Underlie its Roles in Disease and Viral Restriction 94%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.