Swimming motility and chemotaxis control the spatial organization, persistence, and inflammatory activity of a model intestinal pathobiont
Wiles, T. J.; Schlomann, B. H.; Wall, E. S.; Betancourt, R.; Parthasarathy, R.; Guillemin, K.
Show abstract
Understanding the processes that spatially restrict resident gut bacteria and the mechanisms by which disease-causing pathobionts escape this control will open new avenues for microbiome-based therapies. Using live imaging and genetically engineered bacteria, we discovered that flagella-based swimming motility and chemotaxis enable a model Vibrio pathobiont to govern its own spatial organization within the larval zebrafish gut and to persist in the face of the disruptive forces of intestinal flow. Bacterial mutants lacking motility traits became aggregated and lumenally confined, making them susceptible to periodic expulsion from the host. Consequently, non-motile and non-chemotactic mutants experienced large fluctuations in absolute abundance and impaired interbacterial competition. Further, we found that motile bacterial cells induce expression of the proinflammatory cytokine TNF in gut-associated macrophages and the liver. Using inducible genetic switches, we demonstrate that swimming motility can be manipulated in situ to modulate the spatial organization, persistence, and inflammatory activity of gut bacteria.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Hosts Manipulate Lifestyle Switch and Pathogenicity Heterogeneity of Opportunistic Pathogens in the Single-cell Resolution 95%
- Interspecies signaling generates exploratory motility in Pseudomonas aeruginosa 94%
- Pathogen clonal expansion underlies multiorgan dissemination and organ-specific outcomes during systemic infection 94%
Similar papers in this journal
Similar papers in this journal
- A Cell Atlas of Microbe-Responsive Processes in the Zebrafish Intestine 95%
- Early invasion of uropathogenic Escherichia coli into the bladder wall by solitary bacteria that are protected from antibiotics and neutrophil swarms in an organoid model 95%
- Phage-delivered CRISPR-Cas9 for strain-specific depletion and genomic deletions in the gut microbiome 94%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.