Back

Photon-statistics in sensitized emission FRET and FLIM: a comparative theoretical analysis

Esposito, A.

2019-09-18 biophysics
10.1101/774919 bioRxiv
Show abstract

FRET imaging is an essential analytical method in biomedical research. The limited photon-budget experimentally available, however, imposes compromises between spatiotemporal and biochemical resolutions, photodamage and phototoxicity. The study of photon-statistics in biochemical imaging is thus important in guiding the efficient design of instrumentation and assays. Here, we show a comparative analysis of photon-statistics in FRET imaging demonstrating how the precision of FRET imaging varies vastly with imaging parameters. Therefore, we provide analytical and numerical tools for assay optimization. FLIM is a very robust technique with excellent photon-efficiencies but also intensity-based FRET imaging can reach very high precision by utilizing also information within acceptor fluorescence.

Matching journals

The top 4 journals account for 50% of the predicted probability mass.

1
Biophysical Reports
○ 36 papers in training set
Top 0.1%
23.7%
2
Biophysical Journal
○ 545 papers in training set
Top 0.2%
15.5%
3
The Journal of Physical Chemistry Letters
● 58 papers in training set
Top 0.1%
7.5%
4
Scientific Reports
○ 3102 papers in training set
Top 15%
6.6%
50% of probability mass above
5
The Journal of Physical Chemistry B
● 158 papers in training set
Top 0.3%
5.1%
6
ACS Sensors
● 45 papers in training set
Top 0.5%
2.9%
7
Physical Biology
○ 43 papers in training set
Top 0.7%
2.6%
8
PLOS ONE
● 4510 papers in training set
Top 49%
2.0%
9
Journal of Biophotonics
○ 16 papers in training set
Top 0.3%
1.8%
10
Nano Letters
● 63 papers in training set
Top 1%
1.8%
11
Biomedical Optics Express
● 84 papers in training set
Top 0.6%
1.8%
12
eLife
● 5422 papers in training set
Top 40%
1.8%
13
Methods
○ 29 papers in training set
Top 0.2%
1.8%
14
Nature Communications
○ 4913 papers in training set
Top 55%
1.3%
15
The European Physical Journal Plus
○ 13 papers in training set
Top 0.6%
1.0%
16
Analytical Chemistry
● 205 papers in training set
Top 2%
1.0%
17
Chemical Communications
● 24 papers in training set
Top 1%
0.8%
18
International Journal of Molecular Sciences
○ 453 papers in training set
Top 15%
0.8%
19
Biophysics and Physicobiology
● 10 papers in training set
Top 0.2%
0.8%
20
Nucleic Acids Research
◐ 1128 papers in training set
Top 19%
0.7%
21
Communications Biology
○ 886 papers in training set
Top 28%
0.7%
22
Cell Reports Methods
○ 141 papers in training set
Top 6%
0.7%
23
PLOS Computational Biology
● 1633 papers in training set
Top 28%
0.5%
24
Optics Express
● 23 papers in training set
Top 0.5%
0.5%
25
Physical Chemistry Chemical Physics
● 34 papers in training set
Top 0.8%
0.5%
26
ACS Synthetic Biology
● 256 papers in training set
Top 4%
0.5%
27
Biology of the Cell
○ 11 papers in training set
Top 0.3%
0.5%
28
ACS Photonics
● 13 papers in training set
Top 0.5%
0.5%