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Ifnar1 signaling breaks the hepatic urea cycle to regulate adaptive immunity

Lercher, A.; Bhattacharya, A.; Popa, A. M.; Caldera, M.; Schlapansky, M. F.; Baazim, H.; Majek, P.; Brunner, J. S.; Kosack, L. J.; Vitko, D.; Pinter, T.; Guertl, B.; Reil, D.; Kalinke, U.; Bennett, K. L.; Menche, J.; Cheng, P. N.; Schabbauer, G.; Trauner, M.; Klavins, K.; Bergthaler, A.

2019-09-10 immunology
10.1101/762310 bioRxiv
Show abstract

Infections induce complex host responses linked to antiviral defense, inflammation and tissue damage and repair. These processes are increasingly understood to involve systemic metabolic reprogramming. We hypothesized that the liver as a central metabolic hub may orchestrate many of these changes during infection. Thus, we investigated the systemic interplay between inflammation and metabolism in a mouse model of chronic viral infection and hepatitis. Here we show that virus-induced type I interferon (IFN-I) modulates wide-spread metabolic alterations of the liver in a hepatocyte-intrinsic Ifnar1-dependent way. Specifically, IFN-I repressed the transcription of numerous genes with metabolic function including Otc and Ass1, which encode enzymes of the urea cycle. This led to decreased arginine and increased ornithine concentrations in the circulation, resulting in suppressed virus-specific CD8 T cell responses and ameliorated liver pathology. These findings establish IFN-I-induced modulation of hepatic metabolism and the urea cycle as an endogenous mechanism of immunoregulation.

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