CcnA, a novel non-coding RNA regulating the bacterial cell cycle
Beroual, W.; Lalaouna, D.; Benzaina, N.; Valette, O.; Prevost, K.; Denis, Y.; Djendli, M.; Brilli, M.; Robledo, M.; Jimenez Zurdo, J. I.; Masse, E.; Biondi, E. G.
Show abstract
Bacteria are powerful models for understanding how cells divide and accomplish global regulatory programs. In Caulobacter crescentus, a cascade of essential master regulators supervises the correct and sequential activation of DNA replication, cell division and development of different cell types. Among them, the response regulator CtrA plays a crucial role coordinating all those functions. Here, for the first time we describe the role of a novel factor named CcnA, a cell cycle regulated ncRNA located at the origin of replication, presumably activated by CtrA and responsible for the accumulation of CtrA itself. In addition, CcnA may be also involved in the inhibition of translation of the S-phase regulator, GcrA, by interacting with its 5 untranslated region (5-UTR). Performing in vitro experiments and mutagenesis, we propose a mechanism of action of CcnA based on liberation (ctrA) or sequestration (gcrA) of their ribosome-binding site (RBS). Finally, its role may be conserved in other alphaproteobacterial species, such as Sinorhizobium meliloti, representing indeed a potentially conserved process modulating cell cycle in Caulobacterales and Rhizobiales.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- A TetR-family protein activates transcription from a new promoter motif associated with essential genes for autotrophic growth in acetogens 95%
- Identification of the unwinding region in the Clostridioides difficile chromosomal origin of replication 95%
- Internal in-frame translation generates Cas11b, which is important for effective interference in an archaeal CRISPR-Cas system 95%
Similar papers in this journal
- A novel RHH family transcription factor aCcr1 and its viral homologs dictate cell cycle progression in archaea 95%
- The bacterial leader peptide peTrpL has a conserved function in antibiotic-dependent posttranscriptional regulation of ribosomal genes 95%
- Cells with Stochastically Increased Methyltransferase to Restriction Endonuclease Ratio Provide an Entry for Bacteriophage into Protected Cell Population 94%
Similar papers in this journal
- Transcriptional regulation of the Nε-fructoselysine metabolism in Escherichia coli by global and substrate-specific cues 94%
- Multiple mechanisms for overcoming lethal over-initiation of DNA replication 94%
- Genome-wide analysis of in vivo CcpA binding with and without its key co-factor HPr in the major human pathogen group A Streptococcus. 94%
Similar papers in this journal
- An antisense RNA regulates production of DnaA and affects sporulation in Bacillus subtilis 96%
- Inducible expression of (pp)pGpp synthetases in Staphylococcus aureus is associated with activation of stress response genes 94%
- The two-component system ChvGI maintains cell envelope homeostasis in Caulobacter crescentus 93%
Similar papers in this journal
- The interplay between the polar growth determinant DivIVA, the segregation protein ParA and their novel interaction partner PapM controls the Mycobacterium smegmatis cell cycle by modulation of DivIVA subcellular distribution 95%
- Lysis cassette-mediated exoprotein release in Yersinia entomophaga is controlled by a PhoB-like regulator 93%
- Expression of the Bacillus thuringiensis vip3A insecticidal toxin gene is activated at the onset of stationary phase by VipR, an autoregulated transcription factor 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.