Engineering recombination between diverged yeast species reveals genetic incompatibilities
Bozdag, G. O.; Ono, J.; Denton, J. A.; Karakoc, E.; Hunter, N.; Leu, J.-Y.; Greig, D.
Show abstract
The major cause of the sterility of F1 hybrids formed between Saccharomyces cerevisiae and Saccharomyces paradoxus is anti-recombination. The failure of homologous chromosomes from the different species to recombine causes them to mis-segregate, resulting in aneuploid gametes, most of which are inviable. These effects of anti-recombination have previously impeded the search for other forms of incompatibility, such as negative genetic interactions (Bateson-Dobzhoansky-Muller incompatibilities). By suppressing the meiotic expression of MSH2 and SGS1, we could increase recombination and improve hybrid fertility seventy-fold. This allowed us to recover meiotic tetrads in which all four gametes were viable, ensuring that segregation had occurred properly to produce perfectly haploid, not aneuploid, recombinant hybrid gametes. We sequenced the genomes of 84 such tetrads, and discovered that some combinations of alleles from different species were significantly under-represented, indicating that there are incompatible genes contributing to reproductive isolation.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Contrasting mutation patterns in haploid and diploid cells from two yeast species 97%
- Two synthetic 18-way outcrossed populations of diploid budding yeast with utility for complex trait dissection 97%
- CRISPR Mutants of Three Y Chromosome Genes Suggest Gradual Evolution of Fertility Functions in Drosophila melanogaster 96%
Similar papers in this journal
- The Genetic Basis of Differential Autodiploidization in Evolving Yeast Populations 96%
- Comprehensive synthetic genetic array analysis of alleles that interact with mutation of the Saccharomyces cerevisiae RecQ helicases Hrq1 and Sgs1 95%
- Stage-specific disruption of X chromosome expression during spermatogenesis in sterile house mouse hybrids 95%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.