Droplet-digital Cas13a assay enables direct single-molecule microRNA quantification
Tian, T.; Shu, B.; Liu, L.; Zhou, X.
Show abstract
The direct quantification of microRNA at the single-molecule level is still challenging. Herein, we developed a droplet-digital Cas13a assay (ddCA) as a general approach for the amplification-free and absolute quantification of single unlabeled miRNA molecules with single-nucleotide specificity. We demonstrate its simplicity, precise quantification capability, excellent specificity and broad applicability by analyzing microRNAs from synthetic and cell lines-derived materials.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Photon-directed Multiplexed Enzymatic DNA Synthesis for Molecular Digital Data Storage 95%
- Single-Molecule RNA Sizing Enables Quantitative Analysis of Alternative Transcription Termination 95%
- Addressable Nanoantennas with Cleared Hotspots for Single-Molecule Detection on a Portable Smartphone Microscope 95%
Similar papers in this journal
- Dynamic aqueous multiphase reaction system for simple, sensitive and quantitative one-pot CRISPR-Cas12a based molecular diagnosis 95%
- Exceeding 80% efficiency of single-bead encapsulation in microdroplets through hydrogel coating-assisted close-packed ordering 94%
- Sequencing ultra-rare targets with compound nucleic acid cytometry 94%
Similar papers in this journal
- A Cascaded Droplet Microfluidic Platform Enables High-throughput Single Cell Antibiotic Susceptibility Testing at Scale 94%
- Development of single-molecule enzyme activity assay for serine hydrolases using activity-based protein labeling probes 94%
- In vitro neutrophil functional assay in microliter whole blood for days-long extraction of donor-specific information 93%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.