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DUX4 regulates oocyte to embryo transition in human

Vuoristo, S.; Hyden-Granskog, C.; Yoshihara, M.; Gawriyski, L.; Damdimopoulos, A.; Bhagat, S.; Hashimoto, K.; Krjutskov, K.; Ezer, S.; Paluoja, P.; Lundin, K.; Paloviita, P.; Recher, G.; Ranga, V.; Airenne, T.; Tamirat, M.; Jouhilahti, E.-M.; Otonkoski, T.; Tapanainen, J. S.; Kawaji, H.; Murakawa, Y.; Bürglin, T. R.; Varjosalo, M.; Johnson, M. S.; Tuuri, T.; Katayama, S.; Kere, J.

2019-08-12 developmental biology
10.1101/732289 bioRxiv
Show abstract

During the human oocyte-to-embryo transition, the fertilized oocyte undergoes final maturation and the embryo genome is gradually activated during the first three cell divisions. How this transition is coordinated in humans is largely unknown. We show that the double homeodomain transcription factor DUX4 contributes to this transition. DUX4 knockdown in human zygotes caused insufficient transcriptome reprogramming as observed three days after fertilization. Induced DUX4 expression in human embryonic stem cells activated transcription of thousands of newly identified bi-directional transcripts, including putative enhancers for embryonic genome activation genes such as LEUTX. DUX4 protein interacted with transcriptional modifiers that are known to couple enhancers and promoters. Taken together, our results reveal that DUX4 is a pioneer regulating oocyte-to-embryo transition in human through activation of intergenic genome, especially enhancers, and hence setting the stage for early human embryo development.

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