Dynamics of transcription induced by osmostress promoters reveled by live single-cell assays
Wosika, V.; Pelet, S.
Show abstract
Precise regulation of gene expression in response to environmental changes is crucial for cell survival, adaptation and proliferation. In eukaryotic cells, extracellular signal integration is often carried out by Mitogen-Activated Protein Kinases (MAPK). Despite a robust MAPK signaling activity, downstream gene expression can display a great variability between single cells. Using a live mRNA reporter, we monitored the dynamics of transcription in Saccharomyces cerevisiae upon hyper-osmotic shock. The transient activity of the MAPK Hog1 opens a temporal window where stress-response genes can be activated. Here we show that the first minutes of Hog1 activity are essential to control the activation of a promoter. The chromatin repression on a locus slows down this transition and contributes to the variability in gene expression, while binding of transcription factors increases the level of transcription. However, soon after Hog1 activity peaks, negative regulators promote chromatin closure of the locus and transcription progressively stops.
Matching journals
The top 1 journal accounts for 50% of the predicted probability mass.
Similar papers in this journal
- Differential regulation of mRNA stability modulates transcriptional memory and facilitates environmental adaptation 96%
- Machine learning uncovers independently regulated modules in the Bacillus subtilis transcriptome 95%
- Distinct metabolic states of a cell guide alternate fates of mutational buffering through altered proteostasis 95%
Similar papers in this journal
Similar papers in this journal
- The Proxitome of Arabidopsis Processing Bodies Reveals a Condensate-Membrane Interface Instructing Actin-Driven Directional Growth 94%
- ROS production by localized SCHENGEN receptor module drives lignification at subcellular precision 93%
- Pre-meiotic H1.1 degradation is essential for Arabidopsis gametogenesis 93%
Similar papers in this journal
- Yeast cell responses and survival during periodic osmotic stress are controlled by glucose availability 95%
- Transient intracellular acidification regulates the core transcriptional heat shock response 95%
- Live-cell single-molecule tracking highlights requirements for stable Smc5/6 chromatin association in vivo 94%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.