A ciliary BBSome-ARL-6-PDE6D pathway trafficks RAB-28, a negative regulator of extracellular vesicle biogenesis
Akella, J. S.; Carter, S. P.; Rizvi, F.; Nguyen, K. C. Q.; Tsiropoulou, S.; Moran, A. L.; Silva, M.; Kennedy, B. N.; Hall, D. H.; Barr, M. M.; Blacque, O. E.
Show abstract
Cilia both receive and send information, the latter in the form of extracellular vesicles (EVs). EVs are nano-communication devices that cells shed to influence cell, tissue, and organism behavior. Mechanisms driving ciliary EV biogenesis and environment release are almost entirely unknown. Here, we show that the ciliary G-protein RAB28, associated with human autosomal recessive cone-rod dystrophy, negatively regulates EV levels in the sensory organs of Caenorhabditis elegans. We also find that sequential targeting of lipidated RAB28 to periciliary and ciliary membranes is highly dependent on the BBSome and PDE6D, respectively, and that BBSome loss causes excessive and ectopic EV production. Our data indicate that RAB28 and the BBSome are key in vivo regulators of EV production at the periciliary membrane. Our findings also suggest that EVs control sensory organ homeostasis by mediating communication between ciliated neurons and glia, and that defects in ciliary EV biogenesis may contribute to human ciliopathies.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Centrosomal Enrichment and Proteasomal Degradation of SYS-1/-β-catenin Requires the Microtubule Motor Dynein 96%
- Initial ciliary assembly in Chlamydomonas requires Arp2/3 complex-dependent endocytosis 96%
- Interactions between TULP3 tubby domain cargo site and ARL13B amphipathic helix promote lipidated protein transport to cilia 95%
Similar papers in this journal
- Arl13b regulates Sonic Hedgehog signaling from outside primary cilia 96%
- Drosophila Synaptotagmin 7 negatively regulates synaptic vesicle fusion and replenishment in a dosage-dependent manner 96%
- Sticks and Stones, a conserved cell surface ligand for the Type IIa RPTP Lar, regulates neural circuit wiring in Drosophila 96%
Similar papers in this journal
- A role for the Erk MAPK pathway in modulating SAX-7/L1CAM-dependent locomotion in Caenorhabditis elegans 96%
- Functional classification of GNAI1 disorder variants in C. elegans uncovers conserved and cell-specific mechanisms of dysfunction 96%
- Ubiquitin ligases and a processive proteasome facilitate protein clearance during the oocyte-to-embryo transition in Caenorhabditis elegans 95%
Similar papers in this journal
- An extracellular protein regulates patched-related/DAF-6-mediated sensory compartment formation in C. elegans 96%
- Tissue-specific inhibition of protein sumoylation uncovers diverse SUMO functions during C. elegans vulval development 96%
- Caenorhabditis elegans LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells 96%
Similar papers in this journal
- Signaling compartment at the ciliary tip is formed and maintained by intraflagellar transport and functions as sensitive salt detector 97%
- TORC1, BORC, ARL-8 cycling, and kinesin-1 drive vesiculation of cell corpsephagolysosomes 96%
- A sex-specific switch in a single glial cell patterns the apical extracellular matrix 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.