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Enhancing rigor and reproducibility in maternal immune activation models: practical considerations and predicting resilience and susceptibility using baseline immune responsiveness before pregnancy

Estes, M. L.; Farrelly, K.; Cameron, S.; Aboubechara, J. P.; Haapanen, L.; Schauer, J. D.; Horta, A.; Prendergast, K.; MacMahon, J. A.; Shaffer, C. I.; Le, C. T.; Kincheloe, G. N.; Tan, D. J.; van der List, D.; Bauman, M. D.; Carter, C. S.; Van de Water, J.; McAllister, A. K.

2019-07-14 neuroscience
10.1101/699983 bioRxiv
Show abstract

Despite the potential of rodent models of maternal immune activation (MIA) to identify new biomarkers and therapeutic interventions for a range of psychiatric disorders, their value is currently limited by issues of scientific rigor and reproducibility. Here, we report three sources of variability--the immunogenicity of the poly(I:C), the baseline immune responsiveness (BIR) of the females prior to pregnancy, and differences in immune responses in C57/B6 dams across vendors. Similar to the variable effects of human maternal infection, MIA in mice does not cause disease-related phenotypes in all offspring and the magnitude and type of maternal response, determined by a combination of poly(I:C) dose and BIR, predicts offspring outcome. Together, our results provide recommendations for optimization of MIA protocols to enhance rigor and reproducibility and reveal new factors that drive susceptibility of some pregnancies and resilience of others to MIA-induced abnormalities in offspring.

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