Mass Spectrometry based Metabolomics Deciphered Metabolic Reprogramming That Was Required for Biofilm formation in Uropathogenic Escherichia coli
Lu, H.; Que, Y.; Wu, X.; Guan, T.; Guo, H.
Show abstract
Biofilm formation plays a key role in many bacteria causing infections, which mostly accounts for high-frequency infectious recurrence and antibiotics resistance. In this study, we sought to compare modified metabolism of biofilm and planktonic populations with UIT89, a predominant agent of urinary tract infection, by combining mass spectrometry based untargeted and targeted metabolomics methods, as well as cytological visualization, which enable us to identify the driven metabolites and associated metabolic pathways underlying biofilm formation. Surprisingly, our finding revealed distinct differences in both phenotypic morphology and metabolism between two patterns. Furthermore, we identified and characterized 38 differential metabolites and associated three metabolic pathways involving glycerolipid metabolism, amino acid metabolism and carbohydrate metabolism that were altered mostly during biofilm formation. This discovery in metabolic phenotyping permitted biofilm formation shall provide us a novel insight into the desperation of biofilm, which enable to develop novel biofilm based treatments against pathogen causing infections, with lower antibiotic resistance.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Small RNA profiling in Mycobacterium insights into stress adapt ability 94%
- Identification of a Putative Sensor Protein Involved in Regulation of Vesicle Production by a Hypervesiculating Bacterium, Shewanella vesiculosa HM13 94%
- Establishing Essential Oil Stewardship Through the Case of Rosemary and Thyme Oils Against Staphylococcus aureus 94%
Similar papers in this journal
- Biofilm formation displays intrinsic offensive and defensive features of Bacillus cereus 95%
- Evolution of genotypic and phenotypic diversity in multispecies biofilms 93%
- Lifestyle-specific S-nitrosylation of protein cysteine thiols regulates Escherichia coli biofilm formation and resistance to oxidative stress 92%
Similar papers in this journal
- Adaptation of anammox bacteria to low temperature via gradual acclimation and cold shocks: distinctions in protein expression, membrane composition and activities 94%
- L-norepinephrine Induces Community Shift, Oxidative Stress Response, Metabolic Reprogramming, and Virulence Potential in Wastewater Microbiomes 94%
- Distinct growth stages shaped by an interplay of deterministic and neutral processes are indispensable for functional anammox biofilms 93%
Similar papers in this journal
- Exploring the interspecific interactions and the metabolome of the soil isolate Hylemonella gracilis 93%
- A simple, cost-effective and automation-friendly direct PCR approach for bacterial community analysis 93%
- Microbial consortiums of putative degraders of low-density polyethylene-associated compounds in the ocean 93%
Similar papers in this journal
- Cobalt induces the set-up of new structural network in river biofilms: Impairment of autotrophic-heterotrophic coupling. 94%
- Specific quorum sensing molecules are possibly associated with responses to herbicide toxicity in a Pseudomonas strain 93%
- Neonicotinoids stimulate H2-limited methane emission in Periplaneta americana through the regulation of gut bacterium community 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.