N1-methyladenine safeguards against aberrant RNA-protein association during proteostasis stress
Alriquet, M.; Calloni, G.; Martinez-Limon, A.; Delli Ponti, R.; Hanspach, G.; Hengesbach, M.; Tartaglia, G. G.; Vabulas, R. M.
Show abstract
Post-transcriptional modifications of nucleotide bases can effect several aspects of mRNA function. For example, the recent work has established the role of m6A in the coordinated regulation of transcriptome turnover and translation during cellular differentiation and tumorigenesis. The levels of m1A in mRNAs reach up to 10% of those of m6A, yet the functional consequences of this modification are much less clear. Here we show that N1-methyladenine protects mRNAs against aberrant interactions during heat shock and amyloidogenesis in mammalian cells. The m1A methylation motif correlated with the enhanced sequestration of transcripts in stress granules (SG). The cognate methyltransferase TRMT6/61A accumulated and m1A was enriched in SG. Downregulation of the catalytic subunit TRMT61A enhanced amyloidogenesis in the cytosol and increased bystander protein and RNA co-aggregation with A{beta} aggregates. Faulty granulation of mutant RNAs has been implicated in pathogenesis of protein aggregation disorders. Our results demonstrate that also normal mRNAs succumb to co-aggregation with proteins if RNA dynamics during stress is disturbed due to the insufficient N1-adenine methylation.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Phosphorylation regulates arginine-rich RNA-binding protein solubility and oligomerization 95%
- Quantitative proteomics identifies the universally conserved ATPase Ola1p as a positive regulator of heat shock response in Saccharomyces cerevisiae 94%
- PFN2 and NAA80 cooperate to efficiently acetylate the N-terminus of actin 94%
Similar papers in this journal
- ERLIN1/2 scaffolds bridge TMUB1 and RNF170 and restrict cholesterol esterification to regulate the secretory pathway 94%
- An integrated stress response-independent role of GCN2 prevents excessive ribosome biogenesis and mRNA translation 94%
- The type of DNA damage response after Decitabine treatment depends on the level of DNMT activity 94%
Similar papers in this journal
- Large scale discovery of coronavirus-host factor protein interaction motifs reveals SARS-CoV-2 specific mechanisms and vulnerabilities 95%
- SURF2 is a MDM2 antagonist in triggering the nucleolar stress response 95%
- Pptc7 maintains mitochondrial protein content by suppressing receptor-mediated mitophagy 95%
Similar papers in this journal
- p53 isoforms have a high aggregation propensity, interact with chaperones and lack binding to p53 interaction partners 95%
- The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics 94%
- APOE Expression and Secretion are Modulated by Mitochondrial Dysfunction 94%
Similar papers in this journal
- Identification of new interactors of eIF3f by endogenous proximity-dependent biotin labelling in human muscle cells. 94%
- Regulation of PP2A, PP4, and PP6 holoenzyme assembly by carboxyl-terminal methylation 93%
- Tagging allows faithful tracing of expression and enhances biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin. 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.