Dynamics of Meiotic Sex Chromosome Inactivation and Pachytene Activation in Mice Spermatogenesis
Vertesy, A.; Frias-Aldeguer, J.; Sahin, Z.; Rivron, N.; van Oudenaarden, A.; Geijsen, N.
10.1101/665372 bioRxivShow abstract
During germ cell development, cells undergo a drastic switch from mitosis to meiosis to form haploid germ cells. Sequencing and computational technologies now allow studying development at the single-cell level. Here we developed a multiplexed trajectory reconstruction to create a high-resolution developmental map of spermatogonia and prophase-I spermatocytes from testes of a Dazl-GFP reporter mouse. We identified three main transitions in the meiotic prophase-I: meiotic entry, the meiotic sex chromosome inactivation (MSCI), and concomitant pachytene activation. We validated the key features of these transitions in vivo using single molecule FISH. Focusing on MSCI, we found that 34% of sex chromosomal genes are induced shortly before MSCI, that silencing time is diverse and correlates with specific gene functions. These highlight a previously underappreciated level of regulation of MSCI. Finally, we found that spermatozoal genes in pachytene are activated in a temporal pattern reflecting the future anatomic and functional order of the sperm cell. Altogether we highlighted how precise and sequential changes in gene expression regulate cellular states in meiotic prophase-I.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Chromosome Compartments on the Inactive X Guide TAD Formation Independently of Transcription during X-Reactivation 97%
- RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis 96%
- The control of transcriptional memory by stable mitotic bookmarking 96%
Similar papers in this journal
- KRAB-zinc-finger proteins regulate endogenous retroviruses to sculpt germline transcriptomes and genome evolution 97%
- A nuclear role for the Argonaute protein AGO2 in mammalian gametogenesis 97%
- Cell type- and chromosome-specific chromatin landscapes and DNA replication programs of Drosophila testis tumor stem-cell like cells 96%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.