PI4K2β constrains non-canonical Wnt-PCP signalling and is localised by PAR-1 (MARK2/3) phosphorylation

Canonical Wnt signalling is critically important in embryonic cell-type specification and cancer, while non-canonical Wnt signalling is primarily implicated in physical morphogenesis, especially planar cell polarity (PCP). Both are modulated by the polarity kinase PAR-1 (MARK2/3). PAR-1 phosphorylates the Wnt transducer Dishevelled, but there is evidence that it exerts control through other targets. Here we describe an in vitro screen for new targets of PAR-1 in which we identified phosphatidyl-inositol-4-kinase-2-beta (PI4K2{beta}) as a substrate. Perturbation phenotypes and reporter assays in vivo show that PI4K2{beta} inhibits both canonical and non-canonical Wnt pathways, in contrast to PI4K2, which promotes canonical but does not affect non-canonical signalling. We show that PI4K2{beta} acts in Wnt-responding tissue, not in Wnt production or secretion. Subcellularly, PI4K2{beta} is cortically enriched, unlike PI4K2, and is basolateral in polarised cells. Mutation of the PAR-1 phosphorylation site of PI4K2{beta} mis-localises it and the endogenous core PCP protein, Vangl2. Our results reveal that PAR-1 interacts with the vertebrate PCP signalling pathway via PI4K2{beta}.