High-throughput transmission electron microscopy with automated serial sectioning
Graham, B. J.; Hildebrand, D. G. C.; Kuan, A. T.; Maniates-Selvin, J. T.; Thomas, L. A.; Shanny, B. L.; Lee, W.-C. A.
Show abstract
Transmission electron microscopy (TEM) is an essential tool for studying cells and molecules. We present a tape-based, reel-to-reel pipeline that combines automated serial sectioning with automated high-throughput TEM imaging. This acquisition platform provides nanometer-resolution imaging at fast rates for a fraction of the cost of alternative approaches. We demonstrate the utility of this imaging platform for generating datasets of biological tissues with a focus on examining brain circuits.
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