Nup93 and CTCF co-modulate spatiotemporal dynamics and function of the HOXA gene cluster during differentiation
Labade, A. S.; Salvi, A.; Karmodiya, K.; Sengupta, K.
Show abstract
Nucleoporins regulate nuclear transport. In addition, nucleoporins also modulate chromatin organization and gene expression. Here we investigated the role of nucleoporin Nup93, in regulating HOXA gene expression during differentiation. ChIP-Seq analysis revealed that Nup93 associates with genes involved in development and differentiation. Furthermore, Nup93 occupancy significantly overlaps with CTCF. Interestingly, Nup93 and CTCF show antagonistic roles in regulating 3 and 5 end HOXA genes in undifferentiated cells. The HOXA gene locus untethered from the nuclear periphery upon Nup93 but not CTCF depletion, consistent with its upregulation. Remarkably, occupancy of Nup93 and CTCF on HOXA gene locus progressively declined during differentiation but was restored in differentiated cells, consistent with the rerepression and re-localization of the HOXA gene locus with the nuclear periphery upon differentiation. In summary, Nup93 is a key modulator of the spatiotemporal dynamics and function of the HOXA gene locus during differentiation.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Y-complex nucleoporins independently contribute to nuclear pore assembly and gene regulation in neuronal progenitors 96%
- SETD2 negatively regulates cell size through its catalytic activity and SRI domain 95%
- The histone variant macroH2A1.1 regulates RNA Polymerase II paused genes within defined chromatin interaction landscapes. 94%
Similar papers in this journal
- PRDM16 functions as a co-repressor in the BMP pathway to suppress neural stem cell proliferation 95%
- Lin28a/let-7 Pathway Modulates the Hox Code via Polycomb Regulation during Axial Patterning in Vertebrates 95%
- TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation 95%
Similar papers in this journal
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.