Ab-initio discovery of tumoricidal oligonucleotides in a DNA sequencing machine
Mamet, N.; Rusinek, I.; Harari, G.; Shapira, Z.; Zamir, A.; Borovsky, N.; Joseph, N.; Motin, M.; Saban, D.; Bachelet, I.
Show abstract
We describe a technique for the rapid ab-initio discovery of target-tailored tumoricidal DNA oligonucleotides inside an Illumina sequencing chip. By sequencing oligonucleotide pools we generate a physical microfluidic map of hundreds of millions of potential oligo clusters, in which every cluster is mapped to a specific set of spatial coordinates. Tumor cells, pre-loaded with a fluorogenic reporter of apoptosis, are then injected into the chip and monitored over time. Apoptotic tumor cells are identified and analyzed across the entire map, automatically revealing the coordinates of oligos that induced this effect. We demonstrate this method by identifying, within just a few hours, new oligos capable of directly and selectively inducing apoptosis in primary human tumor cells. Such a major capability could lead to a new paradigm of personalized cancer therapy.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Plug-and-play protein biosensors using aptamer-regulated in vitro transcription 96%
- Combi-Seq: Multiplexed transcriptome-based profiling of drug combinations using 1deterministic barcoding in single-cell droplets 96%
- Photon-directed Multiplexed Enzymatic DNA Synthesis for Molecular Digital Data Storage 95%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.