The desmosomal cadherin Desmogon is necessary for the structural integrity of the Medaka notochord
Seleit, A.; Gross, K.; Wölk, M.; Autorino, C.; Centanin, L.; Onistschenko, J.
Show abstract
The notochord is an embryonic tissue that acts as a precursor to the spine. It is composed of outer sheath cells and inner vacuolated cells. Together they ensure the ability of the notochord to act as a hydrostatic skeleton until ossification begins. To date, there is still a paucity in our understanding of how the notochord cell types are specified and the molecular players controlling both their formation and maintenance remain poorly understood. Here we report that desmogon, a desmosomal cadherin, is essential for proper vacuolated cell shape and therefore correct notochord morphology. We trace desmogon+ precursors and uncover an early developmental heterogeneity that dictates the balance of vacuolated and sheath cell formation. We demonstrate that the growth of vacuolated cells occurs asynchronously and reveal the presence of distinct injury sensing mechanisms in the notochord. Additionally, using a small-scale F0 CRISPR screen we implicate uncharacterized genes in notochordal integrity.
Matching journals
The top 2 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Spinal Cord Elongation Enables Proportional Regulation of the Zebrafish Posterior Body 97%
- Coordinated assembly and release of adhesions builds apical junctional belts during de novo polarisation of an epithelial tube 96%
- Versican controlled by Lmx1b regulates hyaluronate density and hydration for semicircular canal morphogenesis 96%
Similar papers in this journal
Similar papers in this journal
- Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach 98%
- Regeneration of the larval sea star nervous system by wounding induced respecification to the sox2 lineage 96%
- Maternally-regulated gastrulation as a source of variation contributing to cavefish forebrain evolution 96%
Similar papers in this journal
- Shared and unique consequences of Joubert Syndrome gene dysfunction on the zebrafish central nervous system 96%
- Dynamic expression and differential requirement of the myocyte fusogen Myomixer during distinct myogenic episodes in the zebrafish 96%
- Segregation of brain and organizer precursors is differentially regulated by Nodal signaling at blastula stage 95%
Similar papers in this journal
- Nuclear translocation of tagged endogenous ERK/MPK-1 MAP Kinase denotes a subset of activation events in C. elegans development 95%
- Dachsous is a key player in epithelial wound closure by modulating cell shape changes and cytoskeleton dynamics 95%
- Interplay between cortical actin and E-cadherin dynamics regulates cell shape in the Drosophila embryonic epidermis 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.