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A new actin depolymerase: a Myosin 1 motor

Pernier, J.; Kusters, R.; Bousquet, H.; Lagny, T.; Morchain, A.; Joanny, J.-F.; Bassereau, P.; Coudrier, E.

2019-10-07 biophysics
10.1101/375923 bioRxiv
Show abstract

The regulation of actin dynamics is essential for various cellular processes. Former evidence suggests a correlation between the function of non-conventional myosin motors and actin dynamics. We investigate the contribution of myosin1b to actin dynamics using sliding motility assays. We observe that sliding on myosin1b immobilized or bound to a fluid bilayer enhances actin depolymerization at the barbed end, while sliding on myosin II, although 5 times faster, has no effect. This work reveals a non-conventional myosin motor as a new type of depolymerase and points to its singular interactions with the actin barbed end.

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