Structural insights into the recognition of mono- and di-acetylated histones by the ATAD2B bromodomain

Bromodomains are chromatin reader modules that recognize acetylated lysine. Different bromodomains exhibit a preference for specific patterns of lysine acetylation marks on core and variant histone proteins, however, the functional relationships that exist between histone acetyllysine ligands and bromodomain recognition remain poorly understood. In this study, we examined the ligand specificity of the ATAD2B bromodomain and compared it to its closely related paralog in ATAD2. We show that the ATAD2B bromodomain selects for mono- and di-acetylated histones, and structural analysis identified key residues in the acetyllysine binding pocket that dictate ligand binding specificity. The X-ray crystal structure of the ATAD2B bromodomain in complex with an ATAD2 bromodomain inhibitor was solved at 2.4 [A] resolution. This structure demonstrated that critical contacts required for bromodomain inhibitor coordination are conserved between the ATAD2/B bromodomains, and many of these residues play a dual role in acetyllysine recognition. We further characterized a variant of the ATAD2B bromodomain that through alternative splicing loses critical amino acids required for histone ligand and inhibitor coordination. Altogether our results outline the structural and functional features of the ATAD2B bromodomain and identify a novel mechanism important for regulating the interaction of the ATAD2B protein with chromatin. HIGHLIGHTSO_LIThe ATAD2B bromodomain recognizes mono- and di-acetylated histone ligands. C_LIO_LIChemical shift perturbations outline the ATAD2B bromodomain acetyllysine binding pocket. C_LIO_LIAn ATAD2B bromodomain-inhibitor complex reveals important binding contacts. C_LIO_LIAn alternate splice variant in the ATAD2B bromodomain abolishes histone and inhibitor binding. C_LI