Immortalization of human nasal and bronchial airway epithelial cells for genome editing applications
van Grinsven, E. J.; Ithakisiou, G. N.; Cleijpool, P.; Bosch, B. M.; Tziouvelis, M.; Amatngalim, G. D.; van Beuningen, S. F. B.; Nijenhuis, W.; Yetkin-Arik, B.; Kapitein, L.; Beekman, J. M.; Akhmanova, A.
Show abstract
In vitro air-liquid interface culture of airway epithelial cells is used as a model system to study respiratory diseases. This culture system not only overcomes the need for animal models or continuous biopsies from individuals but also enables studies of pathophysiology associated with the disease in a patient background. Human airway basal cells serve as progenitor cells for a functional pseudostratified airway epithelium composed mainly of multiciliated and secretory cells. However, due to the limited ability of basal cells to proliferate and differentiate, the long-term use of primary material in culture is restricted. This challenges research that requires genome editing. Here, we describe airway stem cells from nasal and bronchial origin immortalized by hTERT overexpression followed by polyclonal expansion. We demonstrate that this diverse panel of cell lines shows differentiation patterns similar to primary stem cells and can be used for lentiviral and CRISPR/Cas9 genome editing. These cell lines and optimized protocols facilitate airway biology research and disease phenotyping.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Single-cell transcriptomics of dynamic cell behaviors 94%
- GSK3 inhibition rescues growth and telomere dysfunction in dyskeratosis congenita iPSC-derived type II alveolar epithelial cells 93%
- Decoding the IGF1 Signaling Gene Regulatory Network Behind Alveologenesis from A Mouse Model of Bronchopulmonary Dysplasia 93%
Similar papers in this journal
- Pre-existing tissue mechanical hypertension at adherens junctions disrupts apoptotic extrusion in epithelia 91%
- Overlapping roles of JIP3 and JIP4 in promoting axonal transport of lysosomes in human iPSC-derived neurons 91%
- Bridge-like lipid transfer protein family member 2 suppresses ciliogenesis 91%
Similar papers in this journal
- 3D cell culture models demonstrate a role for FGF and WNT signaling in regulation of lung epithelial cell fate and morphogenesis 94%
- Wnt5a and Notum Influence the Temporal Dynamics of Cartilaginous Mesenchymal Condensations in Developing Trachea. 92%
- Primary cilia formation does not rely on WNT/β-catenin signaling 92%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.