Genome-wide CRISPR screen reveals PEX11B as a host restriction factor against ORFV through membrane fluidity regulation
Gao, X.; Hao, J.; Lu, S.; Wang, S.; Sun, Y.; Ke, X.; Gao, X.; Su, Y.; Sun, Y.; Tian, Y.; Yan, W.; Wang, J.; Hai, R.; Zhang, Q.; Wang, J.; Hu, W.; Wang, G.
Show abstract
Host-pathogen interactions are shaped by cellular restriction factors that direct antiviral defenses. We built the first ovine genome-wide CRISPR knockout library in sheep testis (OA3.Ts) cells, targeting all protein-coding genes. Using this platform, we identified PEX11B, a peroxisomal membrane regulatory protein, as a strong restriction factor against orf virus (ORFV) infection. Removing PEX11B increased viral susceptibility and triggered severe cytopathic effects with membrane fusion and syncytia formation. Mechanistic studies showed that PEX11B knockout harmed peroxisomal integrity and disrupted lipid metabolism. This led to greater plasma membrane fluidity, creating a proviral environment that allowed more viral entry and replication. These results reveal a new antiviral function for PEX11B in blocking viral infection and underscore the importance of peroxisomal regulation in host-virus interactions.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- {-}{-}{-}{-}{-}{-}{-}{-}RNA structures within Venezuelan equine encephalitis virus E1 alter macrophage replication fitness and contribute to viral emergence 97%
- Nonlytic cellular release of hepatitis A virus requires dual capsid recruitment of the ESCRT-associated Bro1 domain proteins HD-PTP and ALIX 97%
- Fusogenic Structural Changes in Arenavirus Glycoproteins are Associated with Viroporin Activity 96%
Similar papers in this journal
- The S1/S2 boundary of SARS-CoV-2 spike protein modulates cell entry pathways and transmission 97%
- Spatial and functional arrangement of Ebola virus polymerase inside phase-separated viral factories 96%
- The genetic driver of Acute Necrotizing Encephalopathy, RANBP2, regulates the inflammatory response to Influenza A virus infection 96%
Similar papers in this journal
- In vivo imaging of retrovirus infection reveals a role for Siglec-1/CD169 in multiple routes of transmission 96%
- An arms race between 5'ppp-RNA virus and its alternative recognition receptor MDA5 in RIG-I-lost teleost fish 96%
- Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodies 96%
Similar papers in this journal
- Tunneling nanotubes provide a novel route for SARS-CoV-2 spreading between permissive cells and to non-permissive neuronal cells. 96%
- Zika virus noncoding RNA cooperates with the viral protein NS5 to inhibit STAT1 phosphorylation and facilitate viral pathogenesis 95%
- The SARS-CoV-2 nucleocapsid protein associates with the replication organelles before viral assembly at the Golgi/ERGIC and lysosome-mediated egress 95%
Similar papers in this journal
- A genome-wide genetic screen identified targets for destabilizing the parasitophorous vacuole of Chlamydia trachomatis 96%
- A genome-wide CRISPR screen identifies interactors of the autophagy pathway as conserved coronavirus targets 95%
- Expanded ACE2 dependencies of diverse SARS-like coronavirus receptor binding domains 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.