Protein-Independent Liquid - Liquid Phase Separation of Adenosine Triphosphate under Crowded Conditions
Dec, R.; Dzwolak, W.; Winter, R.
Show abstract
Adenosine triphosphate (ATP), a common constituent of protein-rich biomolecular condensates, itself undergoes liquid-liquid phase separation (LLPS) even in the absence of polypeptides. Using polyethylene glycol as macromolecular crowding agent, we observed robust ATP droplet formation at pH 2-11. Moderate NaCl and lower temperatures promote LLPS by lowering critical ATP and crowder concentrations. Very high salt reverses this trend through anomalous underscreening, but ATP droplets still survive in hypersaline environments. Most importantly, physiological millimolar ATP concentrations are sufficient for phase separation in the presence of millimolar Mg2+ and crowders, mimicking intracellular conditions. pH tunes intermolecular interactions, evidenced by inversion of the adenosine circular dichroism Cotton effect. These results reveal intrinsic, protein-independent LLPS of ATP with potential roles in cellular compartmentalization and pathological phase transitions.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Heterotypic electrostatic interactions control complex phase separation of tau and prion into multiphasic condensates and co-aggregates 94%
- De novo design of modular protein hydrogels with programmable intra- and extracellular viscoelasticity 93%
- SurFlex Microscopy: Measuring Flexibility of Surface-Tethered Biomolecules 93%
Similar papers in this journal
- Mass photometric detection and quantification of nanoscale α-synuclein phase separation 96%
- Non-equilibrium conditions inside rock pores drive fission, maintenance and selection of coacervate protocells 96%
- Phase-specific RNA accumulation and duplex thermodynamics in multiphase coacervate models for membraneless organelles 95%
Similar papers in this journal
Similar papers in this journal
- Non-spherical coacervate shapes in an enzyme driven active system 96%
- Mixtures of Intrinsically Disordered Neuronal Protein Tau and Anionic Liposomes Reveal Distinct Anionic Liposome-Tau Complexes Coexisting with Tau Liquid-Liquid Phase Separated Coacervates 95%
- Amphiphilic protein surfactants reduce the interfacial tension of biomolecular condensates 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.