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Customizable FDM-based zebrafish embryo mold for live imaging

Rivera Pineda, M. X.; Lehtimäki, J.; Jacquemet, G.

2025-11-26 cell biology
10.1101/2025.11.24.689779 bioRxiv
Show abstract

Accurate and reproducible orientation of zebrafish embryos is crucial for high-resolution live imaging but remains challenging with standard agarose mounting. Here, we introduce an orientation tool made using fused deposition modeling (FDM), enhanced with a thin resin coating to improve surface smoothness and performance. This design creates reproducible, embryo-shaped wells that hold larvae in a dorsoventral orientation. The molds, designed to fit standard glass-bottom imaging dishes, are inexpensive to produce on common desktop 3D printers and can be easily modified to fit different imaging setups using the open-source design files. This method enables consistent, long-term live imaging of multiple embryos and broadens the use of low-cost additive manufacturing in zebrafish imaging workflows.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.