FGF13 is not secreted from neurons
Malvezzi, M.; Zhang, H.; Towers, P.; Lyden, D. C.; Marx, S. O.; Pitt, G. S.
Show abstract
FGF13, a noncanonical fibroblast growth factor (FGF) and member of the fibroblast growth factor homologous factor (FHF) subset, lacks a signal sequence and was previously reported to remain intracellular, where it regulates voltage-gated sodium channels (VGSCs) at least in part through direct interaction with the cytoplasmic C-terminus of VGSCs. Recent reports suggest that FGF13 is secreted and regulates neuronal VGSCs through interactions with extracellular domains of integral plasma membrane proteins, yet supportive data are limited. Using rigorous positive and negative controls, we show that transfected FGF13 is not secreted from cultured cells in a heterologous expression system nor is endogenous FGF13 secreted from cultured neurons. Further, employing multiple unbiased screens including proximity protein proteomics, our results suggest FGF13 remains within membranes and is unavailable to interact directly with extracellular protein domains.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- The endocannabinoid 2-arachidonoylglycerol is released and transported on demand via extracellular microvesicles 96%
- Autophagic stress activates distinct compensatory secretory pathways in neurons 95%
- MARK2 phosphorylates KIF13A at a 14-3-3 binding site to polarize vesicular transport of transferrin receptor within dendrites 95%
Similar papers in this journal
- GGA1 interacts with the endosomal Na+/H+ Exchanger NHE6 governing localization to the endosome compartment 95%
- Quantitative interactome proteomics identifies proteostasis network for GABAA receptors 95%
- The C99 domain of the amyloid precursor protein is a disordered membrane phase-preferring protein 95%
Similar papers in this journal
- Oxidative stress induces release of mitochondrial DNA into the extracellular space in human placental villous trophoblast BeWo cells 93%
- Differential Regulation of NHE3 Expression in Type 1 and Type 2 Diabetic Intestine: Impaired Endosomal Regulation of NHE3 Expression in Type 1 Diabetes 92%
- Isolation of functional lysosomes from skeletal muscle 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.