Superoxide dismutase impacts extracellular vesicle biogenesis and uptake
Prova, N. S.; Elsayyid, M.; Tanis, J. E.
Show abstract
Extracellular vesicles (EVs), which transfer bioactive macromolecules between cells, play an important role in the pathogenesis of multiple neurodegenerative diseases. Focus has centered on how altered EV contents propagate disease and the potential for EVs as diagnostic biomarkers, while the effect of pathogenic factors on EV release is less understood. Here, we defined how the key antioxidant enzyme superoxide dismutase 1 (SOD-1) affects EV shedding from sensory neuron primary cilia, enrichment of ciliary proteins packaged into EVs, and uptake of EVs by surrounding glia in vivo by imaging C. elegans expressing fluorescent protein-tagged EV cargos. We discovered that loss of SOD-1, as well as the SOD-1(G85R) amyotrophic lateral sclerosis (ALS) pathogenic variant, increased EV shedding from the cilium distal tip, and this was associated with greater abundance of EV cargo in this ciliary compartment. In contrast, loss of SOD-1 reduced the glial uptake of a different cargo present in EVs shed from the ciliary base. Together, this suggests that redox balance has a subtype-specific effect on EV biogenesis, influencing neuron communication in vivo.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Tracking N-and C-termini of C. elegans polycystin-1 reveals their distinct targeting requirements and functions in cilia and extracellular vesicles 97%
- The CATP-8/P5A-type ATPase functions in multiple pathways during neuronal patterning 96%
- An unexpected role for the conserved ADAM-family metalloprotease ADM-2 in Caenorhabditis elegans molting 95%
Similar papers in this journal
- XDH-1 inactivation causes xanthine stone formation in C. elegans which is inhibited by SULP-4-mediated anion exchange in the excretory cell 94%
- Microbial colonization induces histone acetylation critical for inherited gut-germline-neural signaling 94%
- The Drosophila Amyloid Precursor Protein homologue mediates neuronal survival and neuro-glial interactions 94%
Similar papers in this journal
- Rpgrip1l controls ciliary gating by ensuring the proper amount of Cep290 at the vertebrate transition zone 93%
- Novel structure and composition of the unusually large germline determinant of the wasp Nasonia vitripennis 93%
- Loss of CHMP2A implicates an ordered assembly of ESCRT-III proteins during cytokinetic abscission 92%
Similar papers in this journal
- Large vesicle extrusions from C. elegans neurons are consumed and stimulated by glial-like phagocytosis activity of the neighboring cell 96%
- Lipid homeostasis is essential for a maximal ER stress response 95%
- Forward genetics in C. elegans reveals genetic adaptations to polyunsaturated fatty acid deficiency 94%
Similar papers in this journal
- Light chain 2 is a Tctex-type related axonemal dynein light chain that regulates directional ciliary motility in Trypanosoma brucei 94%
- The purine nucleoside phosphorylase pnp-1 regulates epithelial cell resistance to infection in C. elegans 94%
- A Caenorhabditis elegans nck-1 and filamentous actin-regulating protein pathway mediates a key cellular defense against bacterial pore-forming proteins 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.