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Identification of distinct cDC2 subpopulations that direct microbiota-specific T cell differentiation

Carroll, S. L.; Ly, A.; Liu, A. K.; Canesso, M. C. C.; Victora, G. D.; Mucida, D.; Barton, G. M.

2025-11-05 immunology
10.1101/2025.11.04.686414 bioRxiv
Show abstract

How the complex network of intestinal antigen presenting cells (APCs) instructs CD4+ T cell responses against the microbiota remains unclear. Here, we use Labeling Immune Partnerships by SorTagging Intercellular Contacts (LIPSTIC) to characterize the APCs that prime CD4+ T cells recognizing the commensal bacterium Akkermansia muciniphila. A. muciniphila-specific T cells engaged multiple transcriptionally distinct migratory cDC2 subpopulations, both at homeostasis, when A. muciniphila promotes TFH differentiation, and during inflammation, when it also drives TH1 and TH17 differentiation. The identity of these subpopulations was unchanged by inflammation; however, the distribution of presentation across the subpopulations shifted, with increased presentation by inflammatory cDC2s favoring TH1 and TH17 polarization. These results reveal how distinct T cell differentiation trajectories can be determined through varied interactions with multiple, functionally distinct subpopulations of APCs.

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