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Emergent metabolic parasitism driven by organelle sequestration

Lasek-Nesselquist, E.; Moeller, H. V.; Paight, C.; Seah, B. K. B.; Shaikhutdinov, N.; Swart, E.; Johnson, M. D.

2025-11-05 evolutionary biology
10.1101/2025.11.03.686365 bioRxiv
Show abstract

Stable acquisitions of metabolism, such as the endosymbiotic incorporation of eukaryotic chloroplasts, are thought to proceed through mechanisms that increase the genetic repertoire of the host and allow for vertical integration of new metabolism. Here we test these predictions using the chloroplast-stealing marine ciliate genus Mesodinium by comparing transcriptomes from species that represent a spectrum from full heterotrophy to nearly full phototrophy. In contrast to theory, we find a striking divestment in metabolic autonomy with increased reliance on acquired photosynthesis. Indeed, the highly photosynthetic, red tide-forming Mesodinium rubrum appears to have lost the capacity to synthesize amino acids, metabolize fatty acids, and produce peroxisomes. Our results portray a metabolic parasite, masquerading as a free-living ciliate, yet incapable of satisfying most of its basic anabolic needs.

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