Conditional Gene Expression in Magnaporthe oryzae via Fungal Nitrate Reductase Promoter Replacement
King, J.; Rahnama, M.
Show abstract
Functional genomic studies rely on controlled gene expression, which is challenging for essential genes or those active only under specific conditions. We provide a detailed protocol for generating Magnaporthe oryzae (synonym of Pyricularia oryzae) with conditional BUF1 gene expression. This method uses targeted promoter replacement to substitute the native BUF1 promoter with the nitrogen-responsive promoter. This approach enables nitrogen source-dependent control of expression in M. oryzae, leading to observable pigmentation changes that facilitate easy phenotypic screening.
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