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Quantitative comparison of methodologies for translation site imaging in living cells

Misiaszek, A. D.; Griesbach, E.; Jaugaite, E.; Ortale, A.; Eglinger, J.; Hochstoeger, T.; Chao, J. A.

2025-10-30 biophysics
10.1101/2025.10.29.685314 bioRxiv
Show abstract

Single-molecule imaging of translation sites in living cells has enabled the dynamics of protein synthesis to be investigated with high spatial and temporal resolution. These methodologies utilize the interaction between a multimerized epitope tag and its cognate fluorescent nanobody to detect nascent polypeptides as they emerge from the ribosome. Here, we present a systematic comparison of current methodologies and determine that the ALFA-tag reduces perturbations of mRNA expression and increases the fluorescent signal of translation sites.

Published in RNA · training set

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.