Development of an ex vivo model to study the zoonotic transmission of noroviruses
Villabruna, N.; Hosmillo, M.; Goodfellow, I.; Arnberg, N.; Barcena, J.; Rautenschlein, S.; Beineke, A.; Gerold, G.
Show abstract
Human noroviruses are the most common cause of viral gastroenteritis worldwide, causing sporadic cases and outbreaks. Genetically related animal noroviruses infect wild and domestic animals including pigs and dogs. To investigate the potential of noroviruses to jump the species barrier, we used porcine, canine, and avian precision-cut intestinal slices. We show that human norovirus can bind to pig and dog intestinal tissue and be taken up with a similar efficiency as the porcine and canine noroviruses, respectively. In contrast, no binding or internalization of human, porcine, or canine noroviruses was detected in chicken tissue. We further showed that human norovirus can replicate to some degree in pig intestinal tissue, infecting single cells. In contrast, while animal noroviruses attached to human intestinal cells, intracellular uptake was limited. This suggests that human-to-animal transmission is more likely than animal-to-human transmission and that viral uptake likely presents a species barrier.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Species-specific molecular barriers to SARS-CoV-2 replication in bat cells 95%
- The SARS-CoV-2 Spike is a virulence determinant and plays a major role on the attenuated phenotype of Omicron virus in a feline model of infection 94%
- Mutation nsp6 L232F associated with MERS-CoV zoonotic transmission confers higher viral replication in human respiratory tract cultures ex-vivo 94%
Similar papers in this journal
- A functional interleukin-4 homolog is encoded in the genome of infectious laryngotracheitis virus: unveiling a novel virulence factor 95%
- Rhinovirus C replication is associated with the endoplasmic reticulum and triggers cytopathic effects in an in vitro model of human airway epithelium 94%
- Identification of oncolytic vaccinia restriction factors in canine high-grade mammary tumor cells using single-cell transcriptomics 94%
Similar papers in this journal
- Establishment of a reverse genetic system from a bovine derived Influenza D virus isolate. 95%
- Enteric viruses nucleic acids distribution along the digestive tract of rhesus macaques with idiopathic chronic diarrhea 94%
- Meta-transcriptomic discovery of a divergent circovirus and a chaphamaparvovirus in captive reptiles with proliferative respiratory syndrome 94%
Similar papers in this journal
- Experimental infection of alpacas (Vicugna pacos) with Influenza C and D viruses results in subclinical upper respiratory tract disease 93%
- The cellular Activating Protein 1 cFos regulates Influenza A virus replication 93%
- Hamster and ferret experimental infection with intranasal low dose of a single strain of SARS-CoV-2 93%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.