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PyMOL plugin for Protein Circuit Topology

Dimins, M.; Bazba, A.; Mogyorosi, A.; Kennon, E.; Fiol, T. D.; Hagen, L. A.; Sheikhhassani, V.; Akulov, V.; Mashaghi, A.

2026-08-20 bioinformatics
10.1101/2025.10.21.683762 bioRxiv
Show abstract

Circuit Topology (CT) provides a fundamental framework for analysing folded polymer chains, with applications in functional annotation, protein engineering and drug development. We present a protein CT analysis plugin for PyMOL v3.1.6.1 with a graphical user interface (GUI), automatic installation, and novel features developed through integration with PyMOL's application programming interface (API). The plugin integrates various previously developed CT methodologies for studying structured proteins and their complexes as well as the dynamics of disordered proteins. Analysis of a representative protein and a molecular dynamics trajectory demonstrates the plugin's three analysis modes and their outputs. The plugin reproduces the reference ProteinCT implementation exactly on the structures tested, and is distributed with a versioned release, a pinned environment and a one-command reproduction of every result reported here.

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