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Interpolation-Based Conditioning Of Flow Matching Models For Bioisosteric Ligand Design

Ziv, Y.; Buttenschoen, M.; Scheibelberger, L.; Marsden, B.; Deane, C.

2026-02-16 molecular biology
10.1101/2025.10.20.683377 bioRxiv
Show abstract

AO_SCPLOWBSTRACTC_SCPLOWFast, unconditional 3D generative models can now produce high-quality molecules, but adapting them for specific design tasks often requires costly retraining. To address this, we introduce two training-free, inference-time conditioning strategies, Interpolate-Integrate and Replacement Guidance, that provide control over E(3)-equivariant flow-matching models. Our methods generate bioisosteric 3D molecules by conditioning on seed ligands or fragment sets to preserve key determinants like shape and pharmacophore patterns, without requiring the original fragment atoms to be present. We demonstrate their effectiveness on three drug-relevant tasks: natural product ligand hopping, bioisosteric fragment merging, and pharmacophore merging.

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