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Identity and functions of monoaminergic neurons in the predatory nematode Pristionchus pacificus reveal nervous system conservation and divergence

Loer, C. M.; Yim, H.; Geiger, L. T.; Ramadan, Y. H.; Hampton, M. F.; Bernal, D. V.; Carstensen, H. R.; Morgan, J.; Rivard, L.; Medina, T.; Cook, S. J.; Okumura, M.; Lightfoot, J. W.; Hobert, O.; Hong, R. L.

2025-10-16 neuroscience
10.1101/2025.10.16.682888 bioRxiv
Show abstract

Changes in neurotransmitter usage in homologous neurons may drive evolutionary adaptations in neural circuits across animal phylogeny. The predatory nematode Pristionchus pacificus can be used as a model system to examine nervous system evolution by comparing neurotransmitter expression with that of C. elegans and other nematodes. Here we characterize P. pacificus neurotransmitter expression and function in specific neurons, focusing on its complete set of monoaminergic neurons. We discover patterns of conservation as well as novelties. We examine the roles of monoamines in specific behaviors using neurotransmitter synthesis and vesicular transporter mutants, finding possible differences in the control of host-finding and dispersal behavior.

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