The Arabidopsis CYSTM α 5'UTR increases protein production from transgenes in plants and bacteria.
Khanduja, J. S.; Wu, X.; Searle, I. R.
Show abstract
Achieving high levels of transgene expression is often crucial for the effective production of high value pharmaceutical proteins and bioactive compounds. Here, we show a 79-nucleotide sequence of the 5 UTR of the Arabidopsis thaliana cysteine-rich transmembrane module 1 (CYSTM1) significantly increases transient and stable transgene expression at the post-transcriptional level in multicellular plants, A. thaliana and Nicotiana benthamiana, in monocotyledon wheat germ extracts, and in the unicellular bacterium Escherichia coli by up to 7-fold. We also show the 79 nt CYSTM sequence has 50 % higher transgene expression than the broadly used Tobacco Mosaic Virus omega ({Omega}) sequence in both A. thaliana and N. benthamiana. Taken together, this study provides a short transgene expression enhancer for multicellular and unicellular organisms.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- HY5 is not integral to light mediated stomatal development in Arabidopsis 95%
- GreenGate 2.0: backwards compatible addons for assembly of complex transcriptional units and their stacking with GreenGate 92%
- Identification of gene products involved in plant colonization by Pantoea sp. YR343 using a diguanylate cyclase expressed in the presence of plants 92%
Similar papers in this journal
- Orthogonal control of gene expression in plants using synthetic promoters and CRISPR-based transcription factors 95%
- A heat-shock inducible system for flexible gene expression in cereals 93%
- Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays 91%
Similar papers in this journal
Similar papers in this journal
- Dual-localized enzymatic components that constitute the mitochondrial and plastidial fatty acid synthase systems 94%
- Mechanism of nucleus-chloroplast communication by alternative promoter usage and stromules to establish photomorphogenesis in Arabidopsis 93%
- The PELOTA-HBS1 Complex Orchestrates mRNA Translation Surveillance and PDK1-mediated Plant Growth and Development 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.