A protocol for chemical competence in phytopathogenic Ralstonia
Cowell, T. C.; Guillome, N. R.; Cope-Arguello, M. L.; Prasad, N. N.; Lowe-Power, T. M.
Show abstract
Here, we present a protocol for the transformation of Ralstonia solanacearum species complex (RSSC) strains by calcium chloride-induced chemical competence. We include a step-by-step protocol with media and solution recipes, quantitative results of the protocols efficacy for four of five tested RSSC strains, and an explanation of the logic underlying the protocol development. The protocol involves sequentially treating overnight cultures with a cold calcium chloride solution and a cold calcium chloride/magnesium chloride/glycerol solution, transforming through alternating heat shocks and ice incubations, and recovering cells via an outgrowth in rich medium. Data summarySupplemental File S1 is the preliminary version of this chemical competence protocol. Supplemental Figures S1 and S2 show transformation efficiency data for pUFR80 knockout plasmids, and heat shock number and temperature variation, respectively.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Plasmid-free cheater cells commonly evolve during laboratory growth 94%
- A single nucleotide change in the polC DNA polymerase III in Clostridium thermocellum is sufficient to create a hypermutator phenotype 94%
- Generation of Xylose-inducible promoter tools for Pseudomonas species and their use in implicating a role for the Type II secretion system protein XcpQ in inhibition of corneal epithelial wound closure 94%
Similar papers in this journal
Similar papers in this journal
- One step forward, two steps back: transcriptional advancements and fermentation phenomena in Actinobacillus succinogenes 130Z 95%
- Estimating Microbial Population Data from Optical Density 93%
- Improved transformation efficiency of group A Streptococcus by inactivation of a type I restriction modification system 93%
Similar papers in this journal
- Simple Growth Conditions Improve Targeted Gene Deletion in Cryptococcus neoformans 96%
- The short chain fatty acid propionic acid activates the Rcs stress response system partially through inhibition of D-alanine racemase 93%
- The impact of tropodithietic acid on microbial physiology under varying culture complexities 92%
Similar papers in this journal
- Creatine utilization as a sole nitrogen source in Pseudomonas putida KT2440 is transcriptionally regulated by CahR 94%
- A novel hemA mutation is responsible for small colony variant phenotype in Escherichia coli. 93%
- Development of two compatible plasmids to assess sRNA-mediated post-transcriptional regulation in Acinetobacter baumannii 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.